Alkaline DNA gel running buffer contains 0.5M NaOH and 50 mM EDTA. EDTA is to chelate divalent metal cations such as Mg2+ and Ca2+, which are required for DNA function of degrading enzymes (DNAses).
Alkaline DNA gel running buffer contains 0.5M NaOH and 50 mM EDTA. EDTA is to chelate divalent metal cations such as Mg2+ and Ca2+, which are required for DNA function of degrading enzymes (DNAses).