30% Sucrose, 25 mM Hepes, 60 mM KCl, 2mM EDTA, 0.5 mM EGTA, 0.5 mM Spermine, and 0.15% Spermidine, pH 7.9. Prepared in 3D water and filtered through 0.22 � filter. Shipped at room temperature. Store at 2-8 C.
30% Sucrose, 25 mM Hepes, 60 mM KCl, 2mM EDTA, 0.5 mM EGTA, 0.5 mM Spermine, and 0.15% Spermidine, pH 7.9. Prepared in 3D water and filtered through 0.22 � filter. Shipped at room temperature. Store at 2-8 C.